Antidiabetic Effects of Fenugreek and Cinnamon Extract in Diabetic Mice

Riffat Iqbal; Rabbia Musaddaq; Arslan Habib; Shaista Aslam; Ghulam Jaffar; Zeeshan Ashraf; Muhammad Usman Taj; Muhammad Asif; Jawairia Attique; Maryam Yousaf

Riffat Iqbal Department of Zoology, Government College University, Lahore, Pakistan
Rabbia Musaddaq Department of Zoology, Government College University, Lahore, Pakistan
Arslan Habib Laboratory of Molecular Immunology, School of Life Sciences, Fudan University, Shanghai, China
Shaista Aslam Department of Biological Sciences, National University of Medical Sciences, Islamabad, Pakistan
Ghulam Jaffar Department of Wildlife and Ecology, University of Veterinary and Animals Sciences, Lahore, Pakistan
Zeeshan Ashraf Department of Fisheries and Aquaculture, University of Veterinary and Animals Sciences, Lahore, Pakistan
Muhammad Usman Taj Department of Fisheries and Aquaculture, University of Veterinary and Animals Sciences, Lahore, Pakistan
Muhammad Asif Institute of Continuing Education and Extension, University of Veterinary and Animals Sciences, Lahore, Pakistan
Jawairia Attique Pakistan Institute of Applied and Social Sciences, Kasur, Pakistan
Maryam Yousaf Pakistan Institute of Applied and Social Sciences, Kasur, Pakistan

Keywords: Alloxan, Albino mice, Cinnamon, Fenugreek, Histopathology

Abstract

Background: Diabetes is a life-threatening disease around the globe and causes insulin resistance. Several herbal andallopathic medicines are used against diabetes but some may have fatal side effects. The current study was designed to evaluate the control of hyperglycemia by the use of the combined aqueous extract of cinnamon and fenugreek. Materials and methods: Mice were divided into four groups. Alloxan monohydrate was used to induce diabetes in albino mice with an intraperitoneal dose (200mg/kg body weight). Group 1 (-ve control) consisted of 3 mice with no diabetes, group 2 (+ve control) included 6 mice with diabetes having no treatment, group 3 (herbal treated) contained 6 mice treated with cinnamon and fenugreek orally (200mg/kg) for 21 days (3 weeks) and 6 mice in group 4 were treated with metformin (allopathic). Results: Alloxan monohydrate caused diabetes and an increase in blood sugar level from 98.40±3.89 mg/dl on day 0 to 212.00±12.41mg/dl onday 21 respectively. There was a decrease in body weight from 27.00±0.89gm to 24.42±0.73gm from 0 to 21 days respectively.Blood from alloxan-induced diabetic mice had a high level of urea from 13.2±1.02 to 34.4±1.47 and elevation of creatinine from 0.72±0.058 to 2.88±0.27 from 0 to 21 days respectively. Kidney of alloxan-induced diabetic mice showed marked degenerationof glomeruli with glomerular atrophies. Conclusion: There was a significant decrease in blood sugar, urea and creatinine levels in treated groups of diabetic mice after the administration of extracts.

Author Biographies

Riffat Iqbal, Department of Zoology, Government College University, Lahore, Pakistan

Department of Zoology, Government College University, Lahore, Pakistan

Rabbia Musaddaq, Department of Zoology, Government College University, Lahore, Pakistan

Department of Zoology, Government College University, Lahore, Pakistan

Arslan Habib, Laboratory of Molecular Immunology, School of Life Sciences, Fudan University, Shanghai, China

Laboratory of Molecular Immunology, School of Life Sciences, Fudan University, Shanghai, China

Shaista Aslam, Department of Biological Sciences, National University of Medical Sciences, Islamabad, Pakistan

Department of Biological Sciences, National University of Medical Sciences, Islamabad, Pakistan

Ghulam Jaffar, Department of Wildlife and Ecology, University of Veterinary and Animals Sciences, Lahore, Pakistan

Department of Wildlife and Ecology, University of Veterinary and Animals Sciences, Lahore, Pakistan

Zeeshan Ashraf, Department of Fisheries and Aquaculture, University of Veterinary and Animals Sciences, Lahore, Pakistan

Department of Fisheries and Aquaculture, University of Veterinary and Animals Sciences, Lahore, Pakistan

Muhammad Usman Taj, Department of Fisheries and Aquaculture, University of Veterinary and Animals Sciences, Lahore, Pakistan

Department of Fisheries and Aquaculture, University of Veterinary and Animals Sciences, Lahore, Pakistan

Muhammad Asif, Institute of Continuing Education and Extension, University of Veterinary and Animals Sciences, Lahore, Pakistan

Institute of Continuing Education and Extension, University of Veterinary and Animals Sciences, Lahore, Pakistan

Jawairia Attique, Pakistan Institute of Applied and Social Sciences, Kasur, Pakistan

Pakistan Institute of Applied and Social Sciences, Kasur, Pakistan

Maryam Yousaf, Pakistan Institute of Applied and Social Sciences, Kasur, Pakistan

Pakistan Institute of Applied and Social Sciences, Kasur, Pakistan

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