Methodical analysis of substantial and synthetic equities of lipids and biodiesel extricated from grown Euglena polymorpha

  • Nazim Hussain
  • Dr. Ghazala Yasmeen Butt
  • Mian Shahzada Zia


The research work dealt with biofuel production by extracting lipids from microalgae through trans-esterification process. Now a days third generation biofuel has attained attention world wide to cope up the sharply depleting natural sources of energy. Algae have acquired great consideration as a renewable energy originator due to their synthetic compounds based on lipids extracted from them. Several species of algae had been trialed to investigate the ability of an alga to produce biofuel, under these studies Euglena polymorpha had been tried to produce the biofuel after its invitro culturing in the laboratory. Euglena polymorpha delineated maximal cell growth rate after a week of the culturing. Suitable pH for cell flocculation catalogued 10 at room temperature. In the present analysis, 59.27% of biodiesel was outturned from 0.752 g/l had 29.05% oil contents from the alga. The crude lipid contents of E. polymorpha was found to be 16.33 ± 1.21% of total the dry biomass. Variety of lipids with different saturation magnitudes have been found in the said E. Polymorpha. Saturated and unsaturated fatty acids were retrieved from the experimental alga, plenteous among the monounsaturated fatty acids was Oleaic acid with 19.7% and the least ample was saturated, Methyl caprylate. All the extricated lipids were either saturated or in low of unsaturation magnitude except Linoleic acid which was C19:2 and no polyunsaturated was detected in the scrutinized lipid profile. These lipids were trans-esterified into biodiesel by the process of esterification. Biodiesel procured from the extracted lipids has density 0.816 kg/l and viscosity 3.4 Pa.s at 40 0C. Analysis of saturated fatty acid/Mono-unsaturated fatty acid/Di unsaturated fatty acid revealed that E. Polymorpha could be ideal source for harnessing of biodiesel because it holds predominantly saturated fatty acids and mono-unsaturated fatty acid in most high relative percentages. Furthermore commercial applications must be tried to evaluate its capacity to produce biofuel at the large scale projects.