Detection of surgical wound infections among patients in Azadi teaching hospital in Kirkuk ,using polymerase chain reaction technique

Abstract

Contaminated Surgical wound has been emerged as a problem in many therapy places, which leads to expanded  accommodation period of patients, , making hospitals pay high cost, so, the main goal of this research was to investigate the  microorganisms that could be found in samples that have been drawn from surgical wounds after surgery operations, with examination of antimicrobial  sensitivity  for those organisms, by using polymerase chain reaction (PCR) as a valid method for identifying  isolated microbes , to achieve the research goal ,108 wound swabs have been  collected from patients who had subjected to recent different surgery operations at Azadi teaching hospital in Kirkuk , Iraq , their ages ranged between 10-55 years. Bacterial isolates have been determined through standard microbiological methods. With applying antibiotics sensitivity test for all species of  isolated bacterial, polymerase chain reaction (PCR)  has been conducted to differentiate between these microorganisms, results of this research showed that there are some bacterial species within sites after surgical operations , they have been found in the following order: 35%, 28%, 21%, 8% and 8 % for E. coli, Staphylococcus. aureus,., pseudomonas aeruginosa and Acinatobacter sp., Enterobacter sp., respectively The main microbes that found had a sensitivity  to Amikacin & Vancomycin , ERIC-PCR typing of 17 E. coli and 9 S. aureus isolates, these species were expected to hold different genes depending on their profiles for antimicrobial sensitivity , as it genetically diverse and comprise 4 ERIC-PCR profiles , the DNA fingerprints of these two main bacterial species have been determined and their images were taken . research results could be contribute in more care for wound infections that occur after operations.