Effect of Diode Laser Irradiation on Human Periodontal Ligament Fibroblast

Abstract

Background: Though Diode laser irradiation has been used in various dental treatments, its harmful effects have not much been evaluated especially on human periodontal ligament cells. The aim of this study was to investigate diode laser irradiation effect on the viability and the proliferation of Human Periodontal Ligament Fibroblasts (HPLF). Materials and methods: HPLF cells were cultured in 96-well plates. The cultured cells were then irradiated with various power outputs (0.5W, 1W, 2W and 10W) of diode laser (810nm). The cell viability was determined by MTT assay. The cell proliferation activity of control and irradiated cells were evaluated for seven consecutive days. Results: The result showed comparative cell viability between test and control groups. At day 5, the test group showed significant higher cell proliferation than control group (p<0.05). The 2W group showed the highest cell viability and proliferation activity. Conclusion: The diode laser application in dentistry can be done safely without any alteration in HPLF cells viability, the possibility of stimulating effect on cell proliferation also indicated.