In vitro multiplication of Panagrolaimus sp. (Nematoda: Rhabditida)

Mehrnoush Aminisarteshnizi

Mehrnoush Aminisarteshnizi

Keywords: free-living nematode, Panagrolaimus, goat butter oil, laboratory, multiplication

Abstract

Aim: Artificial culture systems of free-living nematodes can provide much information. Under the controlled condition, life cycles of free-living nematodes influence by temperature and food levels. The aim of the study was to find the best substrate for multiplication of the Panagrolaimus in the laboratory condition.Materials and methods: This study evaluated nine different media for in vitro mass production of Panagrolaimus. The media was including WA 1.5%, WA 1.5% + Goat butter oil, WA 1.5% + K2HPO4, SDA (Sabouraud 4% Dextrose Agar), SDA+ K2HPO4, SDA+ Goat butter oil, NA, NA+ K2HPO4, and NA+ Goat butter oil. The nematodes were counted after four, seven, and fourteen-day. The experiment was repeated two times.Results: Maximum multiplication of all the tested species was observed in WA 1.5% + Goat butter oil as 612.3 after 7 days. These results indicated that goat butter oil was a better medium for the laboratory multiplication of Panagrolaimus.Conclusion: The result indicated that goat butter is a good option to add the media to multiply the free-living nematodes in laboratory conditions.

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